pJF3H
(Plasmid #63897)

• Purpose: For phage display of cDNA libraries
• Depositing Lab: Carlos Barbas
• Publication: Barbas et al Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001. Print.

Backbone

• Vector backbone: pComb3H
• Backbone size w/o insert (bp): 3300
• Total vector size (bp): 3900
• Vector type: Bacterial Expression ; phage display

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cDNA cloning region
• Species: Synthetic
• Insert Size (bp): 200
• Promoter: lacZ
• Tag / Fusion Protein:
  • Fos (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BssHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AAG ACA GCT ATC GCG ATT GCA G
• 3′ sequencing primer: ACC TAT TGC CTA CGG CAG CCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cDNA library insert was provided by Dr. Gregg Silverman while affiliated with the University of California, San Diego. Currently at New York University School of Medicine

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pJF3H utilizes the backbone of pComb3H and a specially designed cassette for cDNA library display. The cDNA library is expressed as a C-Terminal fusion on Fos. The pIII gene product is expressed as a C-Terminal fusion to Jun. The ompA and pelB leader sequences direct the products to the periplasm where they assembly via Jun/Fos interaction.

Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York