Luc-Nanog-3'UTRmut
(Plasmid #63894)

• Purpose: Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated
• Depositing Lab: Lin He
• Publication: Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.

Backbone

• Vector backbone: pGL3-Control
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5256
• Total vector size (bp): 6563
• Modifications to backbone: att L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nanog homeobox
• Alt name: ENK; ecat4; 2410002E02Rik
• Species: M. musculus (mouse)
• Insert Size (bp): 1307
• GenBank ID: 71950 NM_028016.3
• Entrez Gene: Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GLprimer2
• 3′ sequencing primer: RVprimer4

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mir-34a binding site was mutated to remove the seed region