pSG 627
(Plasmid
#63872)
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PurposeMeasurements of translational accuracy
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 4500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelacZ
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SpeciesEscherichia coli
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Insert Size (bp)3200
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MutationUAA near 5' end
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GenBank IDNC_000913.3
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Entrez GenelacZ (a.k.a. b0344, ECK0341)
- Promoter PtacI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NaeI (destroyed during cloning)
- 3′ cloning site ScaI (destroyed during cloning)
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from pSG25
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG 627 was a gift from Albert Dahlberg (Addgene plasmid # 63872 ; http://n2t.net/addgene:63872 ; RRID:Addgene_63872) -
For your References section:
Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity. O'Connor M, Dahlberg AE. Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8. PubMed 8415679