pSG 25
(Plasmid #63867)

• Purpose: Expression of beta-galactosidase at high levels in E. coli
• Depositing Lab: Albert Dahlberg
• Publication: O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.

Backbone

• Vector backbone: pACYC184
• Backbone size w/o insert (bp): 4500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: lacZ
• Species: Escherichia coli
• Insert Size (bp): 3200
• GenBank ID: NC_000913.3
• Entrez Gene: lacZ (a.k.a. b0344, ECK0341)
• Promoter: PtacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NaeI (destroyed during cloning)
• 3′ cloning site: ScaI (destroyed during cloning)
• 5′ sequencing primer: M13pUC-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The lacZ gene originated from pKK-CUG (Bob Weiss; Wagner et al. 1994 J. Bact. 176:1683)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSG25 expresses very high levels of beta-galactosidase in E. coli. Various mutant derivatives are used to measure translational errors.