pSG 415
(Plasmid
#63862)
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PurposeDetect translation initiation from AUC start codon (E. coli lacZ)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 4500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelacZ
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SpeciesEscherichia coli
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Insert Size (bp)3200
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MutationAUC start codon
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GenBank IDNC_000913.3
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Entrez GenelacZ (a.k.a. b0344, ECK0341)
- Promoter PtacI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NaeI (destroyed during cloning)
- 3′ cloning site ScaI (destroyed during cloning)
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from pSG25
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AUG --> AUC start codon in lacZ
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG 415 was a gift from Albert Dahlberg (Addgene plasmid # 63862 ; http://n2t.net/addgene:63862 ; RRID:Addgene_63862) -
For your References section:
Decoding fidelity at the ribosomal A and P sites: influence of mutations in three different regions of the decoding domain in 16S rRNA. O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE. Nucleic Acids Res. 1997 Mar 15;25(6):1185-93. gka224 [pii] PubMed 9092628