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Addgene

pSG 414
(Plasmid #63861)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lacZ
  • Species
    Escherichia coli
  • Insert Size (bp)
    3200
  • Mutation
    UUG start codon
  • GenBank ID
    NC_000913.3
  • Entrez Gene
    lacZ (a.k.a. b0344, ECK0341)
  • Promoter PtacI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NaeI (destroyed during cloning)
  • 3′ cloning site ScaI (destroyed during cloning)
  • 5′ sequencing primer M13pUC-Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from pSG25

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AUG --> UUG start codon in lacZ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG 414 was a gift from Albert Dahlberg (Addgene plasmid # 63861 ; http://n2t.net/addgene:63861 ; RRID:Addgene_63861)

  • For your References section:

    Decoding fidelity at the ribosomal A and P sites: influence of mutations in three different regions of the decoding domain in 16S rRNA. O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE. Nucleic Acids Res. 1997 Mar 15;25(6):1185-93. gka224 [pii] PubMed 9092628
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