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Addgene

pCN-mBFP
(Plasmid #63847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-30
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mBFP
  • Species
    Synthetic
  • Insert Size (bp)
    747
  • Mutation
    Promoter and RBS was swapped. His-tag was removed from pQE-mBFP(Cat-). Ampicillin marker was swapped to Kanamycin.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pBRforEco
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCN-mBFP was a gift from Howard Salis (Addgene plasmid # 63847 ; http://n2t.net/addgene:63847 ; RRID:Addgene_63847)

  • For your References section:

    Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Ng CY, Farasat I, Maranas CD, Salis HM. Metab Eng. 2015 May;29:86-96. doi: 10.1016/j.ymben.2015.03.001. Epub 2015 Mar 10. 10.1016/j.ymben.2015.03.001 PubMed 25769287
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