pTd-4-15-13-3
(Plasmid
#63828)
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PurposeThis plasmid is designed to validate the biophysical model of translational coupling
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFTV3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCFP, mRFP1 and GFPmut3b
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SpeciesSynthetic
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MutationThe intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol . CFPRBS is around 1000 au
- Promoter J23100
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer Cat-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTd-4-15-13-3 was a gift from Howard Salis (Addgene plasmid # 63828 ; http://n2t.net/addgene:63828 ; RRID:Addgene_63828) -
For your References section:
A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons. Tian T, Salis HM. Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 10.1093/nar/gkv635. Epub 2015 Jun 27. 10.1093/nar/gkv635 PubMed 26117546