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Addgene

pTd-4-15-13-2
(Plasmid #63827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FTV3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP, mRFP1 and GFPmut3b
  • Species
    Synthetic
  • Mutation
    The intergenic distance between each gene is -4 nt. The dG_coupling between CFP and RFP is -15kcal/mol, dG_coupling between RFP and GFP is -13kcal/mol . CFPRBS is around 100 au
  • Promoter J23100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Cat-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTd-4-15-13-2 was a gift from Howard Salis (Addgene plasmid # 63827 ; http://n2t.net/addgene:63827 ; RRID:Addgene_63827)

  • For your References section:

    A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons. Tian T, Salis HM. Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 10.1093/nar/gkv635. Epub 2015 Jun 27. 10.1093/nar/gkv635 PubMed 26117546
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