PhLF_N_1
(Plasmid
#63813)
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PurposeNot gate genetic circuit containing one PhlF binding site
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneP15A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePhlF and LacI
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MutationCodon optimized for E.coli
- Promoter pTac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pTac-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PhLF_N_1 was a gift from Howard Salis (Addgene plasmid # 63813 ; http://n2t.net/addgene:63813 ; RRID:Addgene_63813)
