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Addgene

pBad_crtEBI_2
(Plasmid #63803)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FTV
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crtEBI operon
  • Species
    Rhodobactor
  • Mutation
    Codon optimized for E.coli
  • Promoter PBAD

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Needs to be grown in E_IF3_15 strain for overproduction of neurosporene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad_crtEBI_2 was a gift from Howard Salis (Addgene plasmid # 63803 ; http://n2t.net/addgene:63803 ; RRID:Addgene_63803)

  • For your References section:

    Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955. PubMed 24952589
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