SP-dCas9-VPR
(Plasmid #63798)

• Purpose: SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector
• Depositing Lab: George Church
• Publication: Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.

Backbone

• Vector backbone: pcDNA3.3 TOPO
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SP-dCas9-VPR
• Alt name: VP64-p65-Rta
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 5799
• Promoter: CMV
• Tag / Fusion Protein:
  • VPR (VP64-p65-Rta) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • SP-dCas9-VPR
• Articles Citing this Plasmid:
  • 106 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.

This plasmid contains a human codon optimized form of cas9.