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Addgene

pGS1044
(Plasmid #63797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    2710
  • Backbone size w/o insert (bp) 2024
  • Total vector size (bp) 4686
  • Vector type
    E. coli cloning vector
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG followed by KlLEU2
  • Species
    Synthetic
  • Promoter none
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13 rev -49
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ron Prywes (p3xFLAG-S2P; Addgene #32966); derived from plasmid pGS1034

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS1044 was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 63797 ; http://n2t.net/addgene:63797 ; RRID:Addgene_63797)

  • For your References section:

    Dual tagging as an approach to isolate endogenous chromatin remodeling complexes from Saccharomyces cerevisiae. Lin TY, Voronovsky A, Raabe M, Urlaub H, Sander B, Golas MM. Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5. 10.1016/j.bbapap.2014.11.009 PubMed 25486077
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