pGS1034
(Plasmid #63796)

• Purpose: Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker
• Depositing Labs: Monika Golas, Bjoern Sander
• Publication: Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):198-208. doi: 10.1016/j.bbapap.2014.11.009. Epub 2014 Dec 5.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: Fermentas/Thermo
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 5244
• Vector type: E. coli cloning vector
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBP-3xFLAG-TEV-Protein A followed by KlLEU2
• Species: Synthetic
• Insert Size (bp): 2582
• Promoter: none
• Tag / Fusion Protein:
  • CBP-3xFLAG-TEV-Protein A (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: M13 rev -49

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Linda Hicke (LHP1481-TOPO- ProteinA; Addgene #32225) and Ron Prywes (p3xFLAG-S2P; Addgene #32966); Bjoern Sander/Monika M. Golas (pGS1613),

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry