pGS1412
(Plasmid #63787)

• Purpose: Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
• Depositing Labs: Monika Golas, Bjoern Sander
• Publication: Rai et al Mol Biotechnol. 2014 Jun 27.

Backbone

• Vector backbone: pKT175
• Backbone manufacturer: Mark A. Sheff and Kurt S. Thorn
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 4940
• Vector type: E. coli cloning vector
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eCitrine-Strep-tag II followed by URA3 selection marker
• Species: E. coli cloning vector
• Promoter: none
• Tag / Fusion Protein:
  • eCitrine-Strep-tag II (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pQE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mark A. Sheff and Kurt S. Thorn (pKT175)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.