p415GPD-3xHA-GW
(Plasmid
#63781)
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Purpose(Empty Backbone) yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep415GPD
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Backbone manufacturerMumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
- Backbone size (bp) 8652
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Modifications to backboneAdded 3xHA tag and gateway converted
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Vector typeYeast Expression
- Promoter GPD
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Selectable markersLEU2
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Tag
/ Fusion Protein
- 3xHA (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid a modified form of p415GPD from:
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415GPD-3xHA-GW was a gift from Susan Baserga (Addgene plasmid # 63781 ; http://n2t.net/addgene:63781 ; RRID:Addgene_63781) -
For your References section:
A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921
Map uploaded by the depositor.
