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Addgene

p414GPD-3xFLAG-GW
(Plasmid #63780)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63780 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p414GPD
  • Backbone manufacturer
    Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15
  • Backbone size (bp) 7407
  • Modifications to backbone
    Added 3xFLAG tag and gateway converted
  • Vector type
    Yeast Expression
  • Promoter GPD
  • Selectable markers
    TRP1
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid is a modified version of p414GPD from:

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p414GPD-3xFLAG-GW was a gift from Susan Baserga (Addgene plasmid # 63780 ; http://n2t.net/addgene:63780 ; RRID:Addgene_63780)
  • For your References section:

    A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921