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Addgene

pDONR221 Spb1
(Plasmid #63777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 5058
  • Vector type
    Gateway Donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Spb1
  • Alt name
    YCL054W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2523

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221 Spb1 was a gift from Susan Baserga (Addgene plasmid # 63777 ; http://n2t.net/addgene:63777 ; RRID:Addgene_63777)

  • For your References section:

    A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921
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