pDONR221 Mak16
(Plasmid
#63762)
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Purposeyeast ORF Mak16 in the Gateway entry vector pDONR221
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 3453
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Vector typeGateway Donor vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMak16
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Alt nameYAL025C
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)918
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221 Mak16 was a gift from Susan Baserga (Addgene plasmid # 63762 ; http://n2t.net/addgene:63762 ; RRID:Addgene_63762) -
For your References section:
A protein interaction map of the LSU processome. McCann KL, Charette JM, Vincent NG, Baserga SJ. Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. Epub 2015 Apr 15. 10.1101/gad.256370.114 PubMed 25877921