pCVD433
(Plasmid
#63756)
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Purpose(Empty Backbone) Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC184
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Modifications to backboneM1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD433 was a gift from James Kaper (Addgene plasmid # 63756 ; http://n2t.net/addgene:63756 ; RRID:Addgene_63756) -
For your References section:
Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells. Donnenberg MS, Calderwood SB, Donohue-Rolfe A, Keusch GT, Kaper JB. Infect Immun. 1990 Jun;58(6):1565-71. PubMed 2160428
