pMT-TAK1-V5-His
(Plasmid #63753)

• Purpose: Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
• Depositing Lab: Cyril Gueydan
• Publication: pMT-IMD-V5-His (unpublished)

Backbone

• Vector backbone: pMT-V5-His
• Backbone manufacturer: lifetechnologies
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 5582
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TGF-β activated kinase 1
• Alt name: TGF-beta activated kinase 1
• Alt name: dTAK1
• Alt name: dTAK
• Species: D. melanogaster (fly)
• Insert Size (bp): 2035
• Entrez Gene: Tak1 (a.k.a. Dmel_CG18492, CG1388, CG18492, D-TAK, D-tak, D10, DTAK1, DTak, DTak1, DmTak1, Dmel\CG18492, TAK, TAK-1, TAK1, Tak, Tak 1, dTAK, dTAK-1, dTAK1, dTak, dTak1, dtak1, tak, tak1)
• Promoter: pMT
• Tag / Fusion Protein:
  • V5-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CATCTCAGTGCAACTAAA
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry