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Addgene

pMT-TRAF6-V5-His
(Plasmid #63752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-V5-His
  • Backbone manufacturer
    lifetechnologies
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4973
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRAF6
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1427
  • Entrez Gene
    Traf6 (a.k.a. Dmel_CG10961, CG10961, DTRAF2, Dmel\CG10961, EP325, TRAF, TRAF2, TRAF6, Traf2, dTRAF2, dTRAF6, dTraf2, dTraf6, dtraf2, traf2)
  • Promoter pMT
  • Tag / Fusion Protein
    • V5-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CATCTCAGTGCAACTAAA
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-TRAF6-V5-His was a gift from Cyril Gueydan (Addgene plasmid # 63752 ; http://n2t.net/addgene:63752 ; RRID:Addgene_63752)
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