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Addgene

pCVD364
(Plasmid #63739)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 63739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCVD315
  • Backbone manufacturer
    Franklin lab
  • Total vector size (bp) 8700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nanH
  • Species
    V. cholerae
  • Insert Size (bp)
    4800

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBRforEco
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD364 was a gift from James Kaper (Addgene plasmid # 63739 ; http://n2t.net/addgene:63739 ; RRID:Addgene_63739)

  • For your References section:

    Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli. Vimr ER, Lawrisuk L, Galen J, Kaper JB. J Bacteriol. 1988 Apr;170(4):1495-504. PubMed 2832365
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