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Addgene

pCVD315
(Plasmid #63737)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63737 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKO-l
  • Backbone size (bp) 3878
  • Modifications to backbone
    pCVD315 is a derivative of the promoter-screening vector pKO-1 in which bases 1 through 311 have been replaced with the multiple cloning region of M13mpl9RF plus bases 29 through 185 of pBR322. Additional manipulations removed the PstI and AccI restriction sites located outside of the multiple cloning region.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See also Galen et. al. 1990. Advances in Research on Cholera and related Diarrheas, 7, eds., R.B. Sack, Y. Zinnaka p143-153 KTK Scientific Publishers, Tokyo

The copy number of this plasmid construction has increased ca 100 fold from its parent. Reason unknown.

Restriction sites: EcoRI, SstI, KpnI, SmaI/XmaI, BamHI AccI, XbaI, SalI SphI HindIII, ClaI, PstI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD315 was a gift from James Kaper (Addgene plasmid # 63737 ; http://n2t.net/addgene:63737 ; RRID:Addgene_63737)

  • For your References section:

    Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli. Vimr ER, Lawrisuk L, Galen J, Kaper JB. J Bacteriol. 1988 Apr;170(4):1495-504. PubMed 2832365
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