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Addgene

pC2HB
(Plasmid #63734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSN40
  • Backbone size (bp) 9231
  • Vector type
    C. albicans bait plasmid
  • Promoter Met3
  • Selectable markers
    LEU2
  • Tags / Fusion Proteins
    • NLS (N terminal on backbone)
    • LexA (N terminal on backbone)
    • HA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note--the ccdB gene contained is inactive as it is missing the start codon so no ccd survival strain is necessary.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC2HB was a gift from Patrick Van Dijck (Addgene plasmid # 63734 ; http://n2t.net/addgene:63734 ; RRID:Addgene_63734)

  • For your References section:

    A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Stynen B, Van Dijck P, Tournu H. Nucleic Acids Res. 2010 Oct;38(19):e184. doi: 10.1093/nar/gkq725. Epub 2010 Aug 17. 10.1093/nar/gkq725 PubMed 20719741
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