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Addgene

pcDNA3.1(-) FLIPQTV3.0 100micro
(Plasmid #63730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 (-)
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 7528
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPQTV3.0 sensor, 100microM affinity
  • Species
    Synthetic
  • Insert Size (bp)
    2119
  • Mutation
    D157N in glutamine binding domain

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    glnH was amplified by PCR from E.coli genome.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-) FLIPQTV3.0 100micro was a gift from Sakiko Okumoto (Addgene plasmid # 63730 ; http://n2t.net/addgene:63730 ; RRID:Addgene_63730)

  • For your References section:

    Visualization of glutamine transporter activities in living cells using genetically encoded glutamine sensors. Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, Okumoto S. PLoS One. 2012;7(6):e38591. doi: 10.1371/journal.pone.0038591. Epub 2012 Jun 14. 10.1371/journal.pone.0038591 PubMed 22723868
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