MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
(Plasmid #63729)

• Purpose: To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
• Depositing Labs: Mark Kay, Joseph Wu
• Publication: Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.

Backbone

• Vector backbone: no backbone--uses MiP intron for replication in bacteria
• Vector type: Mammalian Expression
• Selectable markers: RNA-OUT

Growth in Bacteria

• Bacterial Resistance(s): RNA-OUT ( 6% sucrose)
• Growth Temperature: 30°C
• Growth Strain(s): RNA-OUT selection competent cells, such as NTC4862 DH5α competent cells (Nature Technology)
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Lin28
• Species: H. sapiens (human)
• Mutation: codon optimized
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: SFFV
• Tag / Fusion Protein:
  • T2A (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: Nanog
• Species: H. sapiens (human)
• Mutation: codon optimized
• Entrez Gene: NANOG

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • annotated sequence MIP339
• A portion of this plasmid was derived from a plasmid made by: The pUC-RNA-OUT sequence used as MIP intron was cloned from NTC vector 8682.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.