pNIC28-Bsa4-iLOVf
(Plasmid
#63722)
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Purpose(Empty Backbone) LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNIC28-Bsa4
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Backbone manufacturerOpher Gileadi
- Backbone size (bp) 7645
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Modifications to backboneInserted translation enhancing element (TEE) and codon optimized iLOV
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- TEE, iLOV, 6xHis, TEV cleavage site (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsT7 polymerase required for expression. Gives very high expression levels, consider reduced (0.1 mM) IPTG for induction and/or reduced induction temperature.
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Copy numberLow Copy
Cloning Information
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCodon optimized iLOV sequence from Andrew Roe & John Christie
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-Bsa4-iLOVf was a gift from Brian Smith (Addgene plasmid # 63722 ; http://n2t.net/addgene:63722 ; RRID:Addgene_63722)
Map uploaded by the depositor.
