BLBP-mCherry
(Plasmid #63721)

• Purpose: Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter
• Depositing Lab: Bart De Strooper
• Publication: Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77. doi: 10.1242/jcs.122440. Epub 2013 Jan 23.

Backbone

• Vector backbone: pCAG-mCherry
• Backbone size w/o insert (bp): 1700
• Total vector size (bp): 5572
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BLBP
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: 1700 bp upstream of BLBP gene cloned in CAG-mCherry
• Entrez Gene: Fabp7 (a.k.a. B-FABP, BFABP, Blbp, MRG)
• Promoter: 1700bp upstream of BLBP gene

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CAATGTCGACAGCACAGCAGAAAGGGAAAA
• 3′ sequencing primer: GGTGGGCGCGCCAGGCAGGAACTGGAGGAACTC

Resource Information

• Supplemental Documents:
  • BLBP-cherry (1.7 kb promoter) genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry