pMSCVpuro-F-Gcn5-NLSm
(Plasmid
#63708)
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Purposeretroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCVpuro
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 8800
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGcn5
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Alt nameKat2a
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Alt nameGcn5l2
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SpeciesM. musculus (mouse)
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Mutationmutated K425, K427 and K438 to R (arginine)
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GenBank IDNM_020004.5
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Entrez GeneKat2a (a.k.a. 1110051E14Rik, Gcn5, Gcn5l2, mmGCN5)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer MSCV5
- 3′ sequencing primer MSCV3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVpuro-F-Gcn5-NLSm was a gift from Kai Ge (Addgene plasmid # 63708 ; http://n2t.net/addgene:63708 ; RRID:Addgene_63708) -
For your References section:
Gcn5 and PCAF negatively regulate interferon-beta production through HAT-independent inhibition of TBK1. Jin Q, Zhuang L, Lai B, Wang C, Li W, Dolan B, Lu Y, Wang Z, Zhao K, Peng W, Dent SY, Ge K. EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. Epub 2014 Sep 30. 10.15252/embr.201438990 PubMed 25269644
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/63/63708/63708-map_evvj4xYGvRq_.pdf.940x940_q85_autocrop.png)