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Addgene

pMSCVpuro-F-Gcn5-NLSm
(Plasmid #63708)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gcn5
  • Alt name
    Kat2a
  • Alt name
    Gcn5l2
  • Species
    M. musculus (mouse)
  • Mutation
    mutated K425, K427 and K438 to R (arginine)
  • GenBank ID
    NM_020004.5
  • Entrez Gene
    Kat2a (a.k.a. 1110051E14Rik, Gcn5, Gcn5l2, mmGCN5)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MSCV5
  • 3′ sequencing primer MSCV3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVpuro-F-Gcn5-NLSm was a gift from Kai Ge (Addgene plasmid # 63708 ; http://n2t.net/addgene:63708 ; RRID:Addgene_63708)

  • For your References section:

    Gcn5 and PCAF negatively regulate interferon-beta production through HAT-independent inhibition of TBK1. Jin Q, Zhuang L, Lai B, Wang C, Li W, Dolan B, Lu Y, Wang Z, Zhao K, Peng W, Dent SY, Ge K. EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. Epub 2014 Sep 30. 10.15252/embr.201438990 PubMed 25269644
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