pMSCVpuro-PCAF
(Plasmid #63705)

• Purpose: retroviral expression of human PCAF in mammalian cells
• Depositing Lab: Kai Ge
• Publication: Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. Epub 2014 Sep 30.

Backbone

• Vector backbone: pMSCVpuro
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 8800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCAF
• Alt name: KAT2B
• Alt name: P300/CBP-associated factor
• Alt name: K(lysine) acetyltransferase 2B
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: E452G
• GenBank ID: BC060823 BC060823
• Entrez Gene: KAT2B (a.k.a. CAF, P/CAF, PCAF)
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: MSCV5
• 3′ sequencing primer: MSCV3

Resource Information

• Supplemental Documents:
  • Sequencing of pMSCVpuro-PCAF plasmid
• A portion of this plasmid was derived from a plasmid made by: MGC clone 71679
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS identified a E452G discrepancy in the PCAF insert relative to Genbank ID: BC060823. It is unknown what, if any, impact this may have on function.