pRetroX GFP Mps1 wt
(Plasmid #63702)

• Purpose: Inducible expression of GFP-Mps1 fusion in mammalian cells
• Depositing Lab: Floris Foijer
• Publication: Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 10.1073/pnas.1400892111. Epub 2014 Sep 2.

Backbone

• Vector backbone: pRetrox
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7305
• Total vector size (bp): 9555
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mps1
• Alt name: TTK
• Alt name: ESK
• Alt name: PYT
• Species: H. sapiens (human)
• Insert Size (bp): 2574
• GenBank ID: Gene ID: 7272
• Entrez Gene: TTK (a.k.a. CT96, ESK, MPH1, MPS1, MPS1L1, PYT)
• Promoter: tight TRE promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: MSCV-rev

Resource Information

• Supplemental Documents:
  • Annotated sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry