pCVD30
(Plasmid
#63691)
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PurposepCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameV. cholera chromosomal DNA
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SpeciesV. cholerae
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Mutation540 bp of CT A is deleted
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Kaper et. al. 1986. Advances in Research on Cholera and Related Diarrheas, 3 eds., S. Kuwahara, N.F. Pierce, 181-191. Copyright 1986 by KTK Scientific Publishers, Tokyo
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD30 was a gift from James Kaper (Addgene plasmid # 63691 ; http://n2t.net/addgene:63691 ; RRID:Addgene_63691) -
For your References section:
A Recombinant Live Oral Cholera Vaccine. Kaper JB, Lockman H, Baldini MM and Levine MM. Nature Biotechnology 2, 345 - 349 (1984) doi:10.1038/nbt0484-345
Map uploaded by the depositor.