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Addgene

pCVD30
(Plasmid #63691)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 63691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    V. cholera chormosomal DNA
  • Species
    V. cholerae
  • Mutation
    540 bp of CT A is deleted

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kaper et. al. 1986. Advances in Research on Cholera and Related Diarrheas, 3 eds., S. Kuwahara, N.F. Pierce, 181-191. Copyright 1986 by KTK Scientific Publishers, Tokyo

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD30 was a gift from James Kaper (Addgene plasmid # 63691 ; http://n2t.net/addgene:63691 ; RRID:Addgene_63691)

  • For your References section:

    A Recombinant Live Oral Cholera Vaccine. Kaper JB, Lockman H, Baldini MM and Levine MM. Nature Biotechnology 2, 345 - 349 (1984) doi:10.1038/nbt0484-345
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