Skip to main content
Addgene

pCVD002
(Plasmid #63688)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only. Availability may be limited outside the U.S. Please log in for more information.
Item Catalog # Description Quantity Price (USD)
Plasmid 63688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBR325
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5996
  • Total vector size (bp) 8000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    enterotoxin genes
  • Species
    V. cholerae
  • Insert Size (bp)
    4000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVD002 was a gift from James Kaper (Addgene plasmid # 63688 ; http://n2t.net/addgene:63688 ; RRID:Addgene_63688)

  • For your References section:

    Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin. Lockman H, Kaper JB. J Biol Chem. 1983 Nov 25;258(22):13722-6. PubMed 6315707
Related items: