-
PurposeAdenoviral vector for XBP1s expression
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 63678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepAdeno-X
- Backbone size w/o insert (bp) 33000
-
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameXBP1
-
SpeciesH. sapiens (human)
-
Mutationspliced
-
Entrez GeneXBP1 (a.k.a. TREB-5, TREB5, XBP-1, XBP2)
-
Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Adeno-X Forward
- 3′ sequencing primer Adeno-X Reverse (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Ad-Flag-XBP1s was a gift from Qingbo Xu & Lingfang Zeng (Addgene plasmid # 63678 ; http://n2t.net/addgene:63678 ; RRID:Addgene_63678) -
For your References section:
Sustained activation of XBP1 splicing leads to endothelial apoptosis and atherosclerosis development in response to disturbed flow. Zeng L, Zampetaki A, Margariti A, Pepe AE, Alam S, Martin D, Xiao Q, Wang W, Jin ZG, Cockerill G, Mori K, Li YS, Hu Y, Chien S, Xu Q. Proc Natl Acad Sci U S A. 2009 May 19;106(20):8326-31. doi: 10.1073/pnas.0903197106. Epub 2009 May 1. 10.1073/pnas.0903197106 PubMed 19416856