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PurposeMammalian expression of Flag tagged HDAC3
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV5-Flag
- Total vector size (bp) 5974
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDAC3
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Alt namehistone deacetylase 3
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SpeciesM. musculus (mouse)
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Entrez GeneHdac3
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5-Flag-HDAC3 was a gift from Qingbo Xu & Lingfang Zeng (Addgene plasmid # 63676 ; http://n2t.net/addgene:63676 ; RRID:Addgene_63676) -
For your References section:
HDAC3 is crucial in shear- and VEGF-induced stem cell differentiation toward endothelial cells. Zeng L, Xiao Q, Margariti A, Zhang Z, Zampetaki A, Patel S, Capogrossi MC, Hu Y, Xu Q. J Cell Biol. 2006 Sep 25;174(7):1059-69. Epub 2006 Sep 18. 10.1083/jcb.200605113 PubMed 16982804
Map uploaded by the depositor.
