pCfB2800
(Plasmid #63657)

• Purpose: pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.
• Depositing Labs: Irina Borodina, Jerome Maury
• Publication: Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016.

Backbone

• Vector backbone: see publication
• Total vector size (bp): 7460
• Vector type: Yeast Expression, Cre/Lox
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP
• Promoter: TEF1 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TEF-seq

Resource Information

• Supplemental Documents:
  • Annotated Genbank file p2800
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. LEU2 477..1562
P K.l. LEU2 1563..2592
loxP 2609..2642
Consensus TY2 3' 2782..2968
pUC ori 3755..4398
AmpR 4501..5359
Consensus TY2 5' 5803..6031
T ADH1 6058..6252
P TEF1 6271..6690
GFP 6700..7416