pCfB2794
(Plasmid #63651)

• Purpose: pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
• Depositing Labs: Irina Borodina, Jerome Maury
• Publication: Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016.

Backbone

• Vector backbone: see publication
• Total vector size (bp): 7460
• Vector type: Yeast Expression, Cre/Lox
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP
• Promoter: TEF1 promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TEF-seq

Resource Information

• Supplemental Documents:
  • Annotated Genbank file p2794

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K. l. URA3 477..1277
P K. l. URA3 1278..1776
loxP 1793..1826
Consensus Ty1Cons2 3' 1966..2171
pUC ori 2945..3588
AmpR 3691..4549
Consensus Ty1Cons2 5' 4993..5224
T ADH1 5251..5445
P TEF1 5464..5883
GFP 5893..6609