pCfB2990
(Plasmid #63645)

• Purpose: (Empty Backbone) pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.
• Depositing Labs: Irina Borodina, Jerome Maury
• Publication: Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016.

Backbone

• Vector backbone: see publication
• Vector type: Yeast Expression, Cre/Lox
• Promoter: none
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Annotated Genbank file p2990

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 257..316
T URA3 317..439
Degradation tag 440..490
K. l. LEU2 491..1576
P K. l. LEU2 1577..2606
loxP 2623..2656
Consensus TY3 3' 2796..2996
pUC ori 3777..4420
AmpR 4523..5381
Consensus TY3 5' 5825..6058
T ADH1 6085..6279