pCfB2989
(Plasmid #63636)

• Purpose: (Empty Backbone) pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.
• Depositing Labs: Irina Borodina, Jerome Maury
• Publication: Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016.

Backbone

• Vector backbone: see publication
• Vector type: Yeast Expression, Cre/Lox
• Promoter: none
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Annotated Genbank file p2989

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 238..297
T URA3 298..420
Degradation tag 421..471
K. l. URA3 472..1272
P_K. l. URA3 1273..1771
loxP 1788..1821
Consensus TY2 3' 1961..2147
pUC ori 2934..3577
AmpR 3680..4538
Consensus TY2 5' 4982..5210
T ADH1 5237..5431