pQE-WT-KIF-R5-IRH
(Plasmid
#63629)
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PurposeK1F-R5-IRH RNAP mutant
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepQE
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF-R5-IRH RNAP
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SpeciesT7 bacteriophage
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MutationL749I, Q754S, R756N, I761V, H772R, and Q786H
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GenBank IDNC_001604.1 NC_001604.1
- Promoter T5/Lac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TTGTGAGCGGATAACAATT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE-WT-KIF-R5-IRH was a gift from Andrew Ellington (Addgene plasmid # 63629 ; http://n2t.net/addgene:63629 ; RRID:Addgene_63629) -
For your References section:
Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry. Meyer AJ, Ellefson JW, Ellington AD. ACS Synth Biol. 2014 Oct 7. 10.1021/sb500299c PubMed 25279711