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Addgene

pQE-WT-CGG-R12-KIR
(Plasmid #63626)

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Item Catalog # Description Quantity Price (USD)
Plasmid 63626 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pQE

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    K1F IKR RNAP
  • Species
    T7 bacteriophage
  • Mutation
    Q744K, L747V, N748H, L749I, R756E, L757M, H772R
  • GenBank ID
    NC_001604.1 NC_001604.1
  • Promoter T5/Lac

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-WT-CGG-R12-KIR was a gift from Andrew Ellington (Addgene plasmid # 63626 ; http://n2t.net/addgene:63626 ; RRID:Addgene_63626)

  • For your References section:

    Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry. Meyer AJ, Ellefson JW, Ellington AD. ACS Synth Biol. 2014 Oct 7. 10.1021/sb500299c PubMed 25279711
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