pGKO2-Gateway
(Plasmid
#63618)
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Purpose(Empty Backbone) a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63618 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKGO2, Addgene plasmid #63617
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Backbone manufacturerYang lab
- Backbone size (bp) 11720
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Modifications to backbone- The ccdB and chloramphenicol resistance genes flanked by the attP sites in pDONR201 (Invitrogen) were amplified by PCR and the resulting product was first cloned in pGEM-T Easy for sequence verification, and subsequently cloned between the XbaI and HindIII sites of pGKO2.
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Vector typeFungal Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGKO2-Gateway was a gift from Seogchan Kang (Addgene plasmid # 63618 ; http://n2t.net/addgene:63618 ; RRID:Addgene_63618) -
For your References section:
A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252
Map uploaded by the depositor.
