pGKO2
(Plasmid
#63617)
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Purpose(Empty Backbone) a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDHt-KS
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Backbone manufacturerKang Lab, Addgene plasmid # 63616
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Modifications to backboneThe modified ChGPD-HSVtk construct (as a blunted EcoRI-HindIII fragment isolated from SK1807) was cloned into a blunted SacI site of pDHt-KS (Blunt ends were generated with T4 DNA polymerase in the presence of dNTPs).
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Vector typeFungal expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We removed some restriction sites from the ChGPD-HSVtk construct via site-directed mutagenesis, and at the same time, improved codons at the mutated sites based on the fungal codon usage.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGKO2 was a gift from Seogchan Kang (Addgene plasmid # 63617 ; http://n2t.net/addgene:63617 ; RRID:Addgene_63617) -
For your References section:
A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252
Map uploaded by the depositor.
