pDHt-KS
(Plasmid
#63616)
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Purpose(Empty Backbone) The multiple cloning site of pGreenII0000 cloned into binary vector pDHt
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDHt
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Backbone manufacturerMullins, E.D., Kang, S., 2001. Transformation: a tool for studying fungal pathogens of plants. Cell. Mol. Life Sci. 58, 2043–205
- Backbone size (bp) 7483
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Modifications to backboneA 300bp PvuII-PvuII fragment of pDHt containing MCS was replaced with a 0.8kb HpaI-StuI fragment isolated from pGreenII0000
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Vector typefungal expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MCS is in reverse orientation as in pDHt-SK
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDHt-KS was a gift from Seogchan Kang (Addgene plasmid # 63616 ; http://n2t.net/addgene:63616 ; RRID:Addgene_63616) -
For your References section:
A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252
Map uploaded by the depositor.
