pGKO1
(Plasmid
#63611)
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Purpose(Empty Backbone) a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63611 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAMBIA1300
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Modifications to backbone1.8 kb EcoRI-HindIII fragment carrying ChGPD-HSVtk was made blunt by treating it with Klenow fragment and dNTPs, and cloned between the XhoI and BstXI sites, which were blunted with T4 DNA polymerase and dNTPs, of pCAMBIA1300 - Note that ligation of blunted HindIII and BstXI sites generated an undesired HindIII site.
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Vector typebinary fungal vector
- Promoter Lac Promoter
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGKO1 was a gift from Seogchan Kang (Addgene plasmid # 63611 ; http://n2t.net/addgene:63611 ; RRID:Addgene_63611) -
For your References section:
A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252
Map uploaded by the depositor.
