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3xFN-Tel-TAL/pCMV-7.1
(Plasmid #63589)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-7.1
  • Backbone manufacturer
    Sigma-Aldrich
  • Backbone size w/o insert (bp) 4623
  • Total vector size (bp) 7416
  • Vector type
    Mammalian Expression, TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFVN-Tel-TAL
  • Alt name
    3xFLAG-tag, V5-tag, NLS, Telomere-TAL
  • Species
    Synthetic
  • Insert Size (bp)
    2793
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG-tag (N terminal on insert)
    • V5-tag (N terminal on insert)
    • nuclear localization signal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not I (destroyed during cloning)
  • 3′ cloning site Pme I (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFN-Tel-TAL/pCMV-7.1 was a gift from Hodaka Fujii (Addgene plasmid # 63589 ; http://n2t.net/addgene:63589 ; RRID:Addgene_63589)

  • For your References section:

    Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171. 10.1038/srep03171 PubMed 24201379
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