pFrt-invCAG-ires-Luc
(Plasmid
#63576)
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PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63576 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC
- Backbone size w/o insert (bp) 1960
- Total vector size (bp) 7215
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Vector typeMammalian Expression, Cre/Lox ; FRT/Flpe recombination mediated insertion
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Selectable markersHygromycin ; if integrated correctly
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
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SpeciesSynthetic
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Insert Size (bp)5255
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFrt-invCAG-ires-Luc was a gift from Ivo Huijbers (Addgene plasmid # 63576 ; http://n2t.net/addgene:63576 ; RRID:Addgene_63576) -
For your References section:
Using the GEMM-ESC strategy to study gene function in mouse models. Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J. Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. Epub 2015 Oct 15. 10.1038/nprot.2015.114 PubMed 26492136
Map uploaded by the depositor.
