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pFastbac1-GST-hUSP7
(Plasmid #63573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP7
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3309
  • Mutation
    Synthetic based on human protein sequence (please see depositor comment section below)
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer Polyhedrin Forward (AAATGATAACCATCTCGC)
  • 3′ sequencing primer SV40 poly A Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastbac1-GST-hUSP7 was a gift from Titia Sixma (Addgene plasmid # 63573 ; http://n2t.net/addgene:63573 ; RRID:Addgene_63573)

  • For your References section:

    Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase. Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK. Mol Cell. 2011 Oct 7;44(1):147-59. doi: 10.1016/j.molcel.2011.06.034. 10.1016/j.molcel.2011.06.034 PubMed 21981925
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