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Addgene

pET3a-hUBA1
(Plasmid #63571)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63571 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBA1
  • Alt name
    Ubiquitin-activating enzyme E1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3087
  • Mutation
    Isoform 2 (1018 amino acids)
  • Entrez Gene
    UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a-hUBA1 was a gift from Titia Sixma (Addgene plasmid # 63571 ; http://n2t.net/addgene:63571 ; RRID:Addgene_63571)

  • For your References section:

    SUMO modification of the ubiquitin-conjugating enzyme E2-25K. Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK. Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. 10.1038/nsmb903 PubMed 15723079
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